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When you click the Starting Point button, you return to the beginning. Type of Access By default, all accesses to the addresses are displayed. When you choose "Selection", you can display, for example, write-only accesses assignment, set, reset.

Since you eliminated the STOP errors in the last exercise, you are now to eliminate the logical errors in this one. In the search for the first logical error you are to become familiar with using the two test functions Monitor block and Monitor variable in combination see slide. What To Do: 1. Enter a variable table with the addresses shown in the slide 2. Activate the test function. In the Editor - without exiting Monitor Variable - monitor FC 16, where the jogging of the conveyor motor is programmed.

Select the size of the windows for the two test functions so that you can arrange them as shown in the slide. The states of the outputs are visible during program execution in the Monitor block test function, while in the Monitor variable test function they are visible at the end of the cycle. Task In the search for the third logical error you are to use the Monitor data block test function, among other things.

Function Check whether the number of transported parts is recorded correctly and Test No. The counting and displaying of the number of transported parts is programmed in FC Therefore, open the FC 19 with the Editor and test it with the Monitor block function.

Correct the FC 19 and retest the function. Find While reference data are displayed, you can start a search for addresses character string in the list displayed at the time. Note The search function is a pure text search, that is, the entries must be "exact - including every dot, dash and space".

This assignment list gives you an overview of which bit is used in which byte of the memory areas input I , output Q and bit memory M and which S5 timer and S5 counter. The type of use reading or writing is not displayed. The inputs I , outputs Q and bit memories M are displayed byte-by-byte in lines. The bits identified with an "x" or binary addresses in the slide, for example, I 1. The colored bytes identify byte, word or doubleword addresses in the slide, for example, input byte IB0, the input word IW2 or the output doubleword QD6 that are used in the user program.

The address dimension byte, word or doubleword comes from the vertical line in one of the columns "B" Byte , "W" Word and "D" Doubleword. Bits that are both colored and have an "x" are used explicitly as a binary address in the user program and are used through a byte, word, or doubleword address. Example see slide : The output Q8. Filter By selecting "Filter", you can choose the memory areas to be listed and restrict the individual address areas.

The same rules as for filtering in the Cross References list apply. These addresses are defined in the symbol table but are not used in the S7 user program. These addresses have been used in the S7 user program but are not defined in the symbol table.

Filter You use "Filter" to make selections of detailed information for the display of unused symbols. Introduction You can compare blocks between online and offline data management or between two user programs on the hard disk of the PG.

With this function, you can determine whether, for example, program corrections were made in the CPU later on and in which network the blocks differ. What To Do 1. With the right mouse button, select the Blocks folder of an S7 program. Select the Compare Blocks menu option.

In the follow-up screen, the blocks that differ are listed. Select the line in which a difference was determined and then select the "Details" button. In the "Compare Blocks - Details" window you can see when the block was modified and if the block length was changed. After you select the "Go To Note Program corrections can only be made in the offline window.

Differences After you select the "Go To If the blocks displayed differ in several locations, you can switch between the different program locations using the "Previous" and "Next" buttons. Example In the example shown above, the FC 17 block which is stored offline in the slide on the left , calls the FC 20 block. For the formal parameter "Display", output Q9. That means, that after the block was downloaded into the CPU, a correction was only made to either the block saved offline or to the block saved online.

You can identify which of the two blocks was modified last by reading out the time stamp in the "Compare Blocks - Results" screen. Function and When a CPU transitions to STOP, all digital outputs are switched off and analog Area of Use outputs are switched to their defined parameter - either off, as is, or a predefined value.

The "Enable Peripheral Outputs" function is used mainly to check peripheral output wiring. It can, however, also be used to continue to control actuators in the process, even though the CPU has gone into the STOP mode because an error has occurred. What To Do To enable the peripheral outputs, proceed as follows: 1. Open or edit a variable table VAT that contains the peripheral outputs that you want to test or modify specify the peripheral outputs byte-by-byte, word-by-word or doubleword-by- doubleword; you cannot modify individual output bits!

Enter the appropriate values for the peripheral outputs you want to modify in the "Modify Value" column. Function and With Force, you can overwrite variables with any values you like, independent of Area of Use the user program.

Selecting the 1. The status bar also shows the date and time of the current force job in the CPU. If no address in the CPU is forced, this window is empty. In the "Address" column enter the variables and in the "Force Values" column enter the values you want.

Note The following pages contain either further information or are for reference to complete a topic. Test Operation In test operation, all test functions can be performed without restrictions. The status of programmed loops is determined every time they are executed. Process Operation In process operation, the test functions are restricted in such a way that the permissible scan cycle time increase that you select in the CPU parameter assignment is not exceeded.

As well, the status Accumulator 1, Accumulator 2 etc. With Call Environment, you can specify which call or which execution of the block is to be monitored. The Call-up Path can have up to three nesting depths or up to three blocks.

Thus, the 3rd. Example in the slide on the left hand side : FC 99 status block is to be monitored when it is called from FC 70 3rd. Example in the slide on the right hand side : FB 12 status block, not visible in the slide is to be monitored when it is called with Instance data block DB 7. That is, the call-up path for all three FC 99 calls is the same. Accordingly, it is not possible to specifically call one of the FC 99 calls by defining a call-up path as trigger condition.

A triggering using Open Data Blocks is also not possible since neither a global nor an instance DB is relevant with the FC 99 calls shown. Task In the search for this logical error you are to use the Monitor block test function with triggering of a specific block call. Function Check whether the occurrence of Disturbance 3 Switch I 1. After Disturbance 3 occurs, no flashing light is displayed.

Since it concerns a parameter, the error is not in the FB 20 itself, but in the actual parameters that are passed on to the FB 20 by the calling block. The FB 20 is called in several locations in the program. Correct the FB 20 call in the calling block and retest the evaluation of the disturbance function. Breakpoints With the help of this test function, you can test a program you see in STL in single- step mode and thus follow the sequence of the executed instructions as well as the associated register contents.

You can set several breakpoints, depending on the CPU. The number of possible breakpoints depends on the CPU used. Note In order to carry out these test functions, you must have fulfilled the following requirements:. Breakpoint You can choose the breakpoint functions in the Program Editor by selecting the Functions "Test" menu option or through the Breakpoint Bar. The outputs are deactivated for safety reasons. Execute Call. Breakpoints Active With "Breakpoints Active" you activate all breakpoints; not only those already set but also those still to be set.

Resume With "Resume", the program runs until the next active breakpoint. Next Statement With "Next Statement", you execute the program in single-step. If you reach a block call, you jump to the first statement after the block call with "Next Statement". The Execute Next Statement and Execute Call menu options require a free breakpoint for the internal implementation.

Execute Call Here, when you reach a block call you branch into the block with "Execute Call". At the end of the block you jump back to the next statement after the block call. Open navigation menu.

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Ankit Maini. We tested the bone marrow stromal cells and enriched fibroblasts from ten normal sub- jects and three DBA patients on Me cells 0 ,Ol ,1 1 10 loo lo00 [lo]. Ten p g of anti-SCF polyclonal antibody is able to neutral- Fig. Me cell line growth response to different ize five ng di rhSCF Fig. In contrast, erythroid prog- enitor proliferation and differentiation occurs only after addition of SCF in vitro, which is able to induce a dramatic increase of both number and size of erythroid burst colonies.

SCF production Me bioassay from unstim- cytokines [16]. Patients with DBA [ have macrocytic 1 anemia with several hematological abnormali- ties, similar to those of Wand Sl mutant mice [ Studies on the in vitro growth of bone mar- row cells from DBA patients demonstrated that granulocytic and megakaryocytic progenitors are able to form colonies in cultures stimulated by several cytokines in a way similar to normal Table HC seems to be Council grant no.

The in vitro effect of to G. Bagnara, G. Paolucci and L. Identification, purification and marrow microenvironment by DBA patients is biological characterization of hematopoietic stem normal. Since normal amounts of IL-3 have cell factor from Buffalo rat liver-conditioned been found to be produced by phytohemagglu- medium. Cell ; Molecular involved in erythroid impairment in DBA. SJ, Suggs SV. Stem cell factor is encoded at the Sl However, the structural analysis of SCF does locus of the mouse and is the ligand for the c-kit not in itself reveal its biological activity on tar- tyrosine-kinase receptor.

Papayannopoulou T. SCF receptors are erythroid progenitors to the ligand for c-kir. Blood distributed among granulocytic, megakary- ; Blood Several biological aspects of SCF-pro- ; DBA pathogenesis. Blood ; Molecular Cloning. Blood ; Long-term culture of human bone marrow cells. Genomic sequencing. Single cytopenias. Hematology of Infancy and Childhood.

Philadelphia: W. Saunders Co. Tura S, Pignatti PF. Host origin of bone marrow 15 Russel ES. Hereditary anemias of the mouse: a fibroblasts following allogeneic bone marrow review for geneticists.

Adv genet ; transplantation for chronic myeloid leukemia. Bone Marrow Transplant ; Me human human hematopoiesis. Int J Cell Cloning leukemic factor-dependent cell line provides a ; Single step method marrow fibroblasts. Blood ;78 suppl 1 a. Anal Biochem



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